Molecular Biophysics of DNA repair nanomachines
Centro Nacional de Biotecnología (CSIC)
C/Darwin 3, Campus de Cantoblanco 28049 Madrid, Spain
We are interested in studying dsDNA break repair and Chromosome organisation at
the single molecule level using Atomic Force Microscopy, Magnetic
and Optical Tweezers, and standard biochemical techniques.
Looking for a lab to do your Master or PhD theses?
We have very interesting projects for physiscists, and biologists in their last year of their degree. Requiered: a good academic record, and high level of English. Contact: F.Moreno-Herrero
Flyer (in Spanish). Flyer (in English)
IX Spanish Fuerzas y Tunel Conference
27-29 AUGUST 2014
San Sebastian, Spain
Organizers: F. Moreno-Herrero and C. Rogero
XX International Summer School Nicolas Cabrera
BIOMOLECULES AND SINGLE MOLECULE TECHNIQUES
21-26 JULY 2013
Miraflores de la Sierra, Madrid, Spain
JULY. We welcome L. Sanchez as a summer JAE-Intro student
JUNE. New paper accepted in PNAS on the AddAB scanning mechanism
JUNE. M.E. Fuentes-Perez. Talk at the Spanish Biophysical Society Meeting, Valencia
JUNE. B. Gollnick. Talk at the Spanish Biophysical Society Meeting, Valencia
JUNE. New paper accepted in ACSnano on DNA origami nanopores
MAY. New paper accepted in ACSnano on peptide-DNA interactions
MAY. We welcome V.Casina for a week as a visiting researcher
MARCH. New paper accepted in Lab on a Chip on DNA origami
FEB. New paper accepted in METHODS on volumetric AFM methods
FEB. C. Carrasco. Internship at S. Leuba and S. Khan laboratories, Pittsburg
FEB-MARCH. B. Gollnick. Internship at U. Keyser lab, Cambridge.
FEB. C. Carrasco. Talk at the Biophysical Society Meeting, Philadelphia
JAN. A. Karlowicz intership at the lab for AFM experiments.
DEC. F. Moreno visits M. Dillingham lab.
DEC. M.E. Fuentes visits M. Dillingham lab.
DEC. New paper accepted in JACS on mechanical properties of dsRNA.
NOV.The M-H Lab collaborates with the Dresden Nanosaurs Team in
Biomod2012 competition. Learn about their project here.
Congratulations for a second overall position in the Biomod 2012 contest!!! LINK.
SEPT. Sanford Leuba and Saleem Khan visit the lab and give a talk.
SEPT. ME. Fuentes and F. Moreno at Spanish AFM and STM conference, El Escorial.
SEPT. F.Moreno receives the IZASA-Werfen prize of the SEBBM. Photo
SEPT. F. Moreno talk at the IUBMB-FEBS-SEBBM conference, Sevilla.
AUG. F. Moreno talk at the 73 Harden Conference - Machines on Genes II, Oxford.
AUG. Kasia Wegrzyn visits the lab for AFM experiments.
JULY. F.Moreno and C. Carrasco. Poster at GRC on Single Molecule Approaches to Biology, Vermont USA.
JULY. Kasia Wegrzyn visits the lab for AFM experiments.
JULY. C. Carrasco visits Sanford Leuba Lab at Univ. of Pittsburg and give a talk.
JULY. F. Moreno. Poster at the Spanish Biophysical Society Meeting, Barcelona.
JULY. B. Gollnick. Talk at the Spanish Biophysical Society Meeting, Barcelona.
JUNE. C. Carrasco visits Ivan Schaap Lab at Georg August Universitat and give a talk.
JUNE. Annual Summer Lunch Party.
JUNE. Marian Oliva visits the lab for AFM experiments.
MAY. Claire Wyman visits the lab and give a talk.
MAY. Erik Schaeffer visits the lab and give a talk.
MAY. F. Moreno organizes the II Workshop on Nanobioscience CNB-CSIC-IMDEA-Nanociencia in Madrid.
MAY. F. Moreno wins the 2012 IZASA-WERFEN prize of the Spanish Biochemical and Molecular Biology Society.
APRIL. F. Moreno gives an invited talk at the 3rd European Nanomanipulation Workshop, Madrid.
APRIL. James Talylor visits the lab for AFM experiments.
APRIL. ERC Annual Project reunion in Bristol.
MARCH. Silvia Hernandez visit the lab for AFM experiments
MARCH. F. Moreno. Visit Frank Sobbot Lab in Antwerp
FEB. C. Carrasco. Talk at the Biophysical Society Meeting, San Diego
FEB. F. Moreno. Visit M. Dillingham lab in Bristol
FEB. Hergen Brutzer visits the lab and gives a talk
FEB. ME. Fuentes. Poster at Linz Winter Workshop
JAN. We welcome Francesca Zuttion as Master Student
DEC. F. Moreno Lab secure a CSIC I-LINK project (2012-2013)
OCT. We welcome MT. Arranz as Final Project Student.
OCT. F. Moreno. Poster at Current Opinion Conference in Amsterdam.
OCT. F. Moreno. Talk at Prof. C. Dekker and N.H. Dekker Labs (Delft).
OCT. F. Moreno. Invited talk at Prof. F. Ritort lab (Barcelona).
SEP. We welcome C. Pastrana as Master Student.
SEP. F. Moreno Lab secure a MICINN project (2012-2014)
AUG. F. Moreno. Invited talk at Dr. Ralf Seidel lab at Dresden.
AUG. C. Carrasco. Talk at the EBSA Conference.
AUG. B. Gollnick. Best Poster Award at the EBSA Conference.
JUNE. ME. Fuentes. Talk at the Spanish Biophysical Society Conference.
JUNE. B. Gollnick. Best Poster Award at the Spanish Biophysical Soc. Conference.
JUNE. F. Moreno. Invited talk at the Spanish Biophysical Society Conference.
JUNE. ERC Annual Project reunion in Madrid.
JUNE. Molecular Cell paper in press.
MAY. Dr. Ralf Seidel visits the lab and gives a talk.
APRIL. Biophysical Journal papers in press.
Day 21, and their livers were analyzed for total porphyrin content as described in refs. 8 and 17. The activity of uro-d was measured as described below. Cytosolic uro-d protein was estimated by separating 30 î¼g of cytosolic protein on a 12% polyacrylamide gel followed by immunoblotting with a rabbit anti-human uro-d polyclonal antiserum as previously described (43). buy viagra Protein concentrations were measured by using the bca reagent (pierce, rockford, il). Uro-d activity assays. Activity of uro-d present in cytosolic extracts prepared from 1. 5 g of mouse liver homogenized in 4 vol of 0. buy generic viagra online 25 m sucrose was assayed as previously described (8, 19). Processing of cytosolic extracts was carried out under minimal illumination by using darkroom safelights. buy viagra cheap Hepatic cytosol (250 î¼l) was denatured by heating (100â°c, 30 min) and clarified by centrifugation (14,000 ã— g) for 10 min. Inhibitory activity of the processed extract was assayed in a 200-î¼l mixture containing 70 î¼l of processed extract, 0. 4 î¼g of rhuro-d, and 6. 0â€“6. 5 nmol of uroporphyrinogen i (8, 13). Hydroxymethylbilane was enzymatically generated from 276 î¼m pbg (frontier scientific) with 3â€“4 î¼g of purified pbg-d in the presence of 7. where to buy viagra in london over the counter 5 mm dtt and 0. 1 m tris, ph 7. buy viagra canada 65 (total volume c î¼l), for 30 min (19). The uroporphyrinogen i macrocycle is rapidly formed by nonenzymatic closure of the linear hydroxymethylbilane. buy cheap viagra Uroporphyrinogen iii was generated by addition of 2â€“3 î¼g of purified recombinant human u3s (44) to the above reaction mixture. Each substrate-generating mixture was incubated for 30 min at 37â°c, neutralized with 20 î¼l of 0. physician samples of viagra 15 m potassium phosphate, and added to the processed extract and rhuro-d. viagra coupon The rhuro-d activity assay mixture was then incubated for 30 min at 37â°c. The processed extract was replaced with buffer as a control for uninhibited rhuro-d activity. where to buy viagra in london over the counter Porphyrins present in the processed extract were measured by replacing the substrate-generating mixture and rhuro-d with buffer. Reactions were stopped by adding an equal volume (200 î¼l) of 3 m hcl and then illuminated under uv light for 30 min to convert all reaction products to porphyrins. Porphyrins were then separated and quantified by hplc as previously described (19). most effective viagra or viagra U3s assays. U3s assays were performed by using our previously described method (44), using aliquots of cytosolic extract in place of recombinant human u3s. Isolation of the inhibitor. The inhibitor in processed extracts was partially purified by solid-phase extraction by using an oasis hlb cartridge (waters, milford, ma) preconditioned with methanol followed by 2% (vol/vol) acetic acid. watermelon rind natural viagra The extract was acidified to ph 4. viagra uk men 0. MARCH. F. Moreno. Poster at BPS, Baltimore.
FEB. First papers accepted as independent researcher.
DEC. F. Moreno talks at Cavendish Lab.(Cambridge) Host: Dr. Keyser.
DEC. F. Moreno and B.Gollnick visit NIMR. London.
DEC. Dr. G. Lynch visit the lab and give a talk.
JULY. B.Gollnick. Poster at Spanish Biophysical Society Conference.
JUNE. C.Carrasco. Poster at Gordon Conferece.
MAY. ERC Annual Project reunion in Bristol.
MAR. Dr. Nynke Dekker visit the lab and give a talk.
FEB. F. Moreno. Poster at BPS, San Francisco.
FEB. AFM course at Univ. at Milano. Link out.
JAN. F. Moreno talks at the IMM-CSIC, Tres Cantos.
JAN. F. Moreno talks at Biomed Institute at Valencia.
DEC. F. Moreno talks at The Annual Winter Conference at the CNB-CSIC, Madrid.
DEC. We welcome M. E. Fuentes as a Ph.D. Student.
NOV. Conference in Modena. Link out.
NOV. We welcome C. Carrasco as a Postdoctoral Researcher.
SEPT. Spanish Biochemical and Molecular Biology Conference. Oviedo, Spain.
SEPT. F. Moreno appointed as Cientifico Titular CSIC at the CNB, Madrid.
JULY. We welcome M. Meunier as a visiting student from Institut d´optique at Paris.
JULY. ERC Annual Project reunion in Madrid.
JUNE. Electron Microscopy Conference at Segovia, Spain
MAY. F. Moreno posters at a Conference at the CBM-CSIC, Madrid
APRIL. Moreno-Herrero Lab kick-off at the CNB-CSIC, Madrid
DEC. F. Moreno and B. Gollnick visited the Dillingham Lab at Bristol.
OCT. We welcome B. Gollnick as Ph.D. Student.
JAN. F. Moreno: ERC Starting Grant Winner Link out.